Manual Therapy, Posturology & Rehabilitation Journal
Manual Therapy, Posturology & Rehabilitation Journal
Research Article

Analysis of low level laser therapy in vitro cultures of bacteria and fungi

Andraus, Rodrigo Antonio Carvalho; Maia, Luciana Prado; Santos, João Paulo Manfré dos; Mesquita, Amanda Rezende; Santos, Thaynara Gonzaga; Braoios, Alexandre; Prado, Rodrigo Paschoal

Downloads: 1
Views: 695


Background: Low intensity laser therapy has been used to accelerate the ulcers healing due to its healing, anti-inflammatory, analgesic and anti-edematous effects. Contaminations by bacteria or fungi on these ulcers commonly happen, constituting a major complication of healing, but there is no consensus about laser therapy bactericidal or fungicidal effect. Aim: To analyze the effects of different times of appliantion using the 808nm and 660nm lasers on cultures of different bacterial species and fungi in vitro. Method: Photon Lase III - DMC was used, with the wavelengths of 660 and 808nm and 30mW, 60mW and 100mW of power, with different exposure time to laser irradiation. Fifteen Petri plates were prepared, 12 plates containing Gram-positive (3 Staphylococcus aureus plates, 3 nterococcus plates) and gram negative bacterias (3 Escherichia coli, 3 Klebsiella pneumonia plates) and 3 Petri plates with the fungi Candida albicans. Plates were randomly divided into 3 groups with 5 plates each group. Plates of Group 1 were irradiated with 660 nm laser at nine different points (A, A 1 , A 2 /B, B 1 , B 2 /C, C 1 , C 2 ), and points A/B/C reciebed only one application, points A 1 ,B 1 ,C 1 received 2 applications and points A 2 ,B 2 ,C 2 received 3 applications. Plates from group 2 where irradiated with 660nm also in nine diferente points, following the same criterea of group 1, while plate of group 3 where used as control, without irradiation. Groups 1 and 2 were irradiated with 144 J/cm2 dose at point A (irradiation time of 2 minutes and 15 seconds), point B (irradiation time of 1 minute 7 seconds) and at point C (40 seconds). After the irradiations the plates were incubated for 24 hours. The cultures were visually examined to check the presence or not of the inhibition zone. In all the groups, no zone of inhibition nor growth were observed. Conclusion: Low intensity laser therapy


Bacteria, Low intensity laser therapy, Infection, Fungi.


1. Diegelman RF, Evans MC. Wound Healing: An Overviewof Acute, Fibrotic and Delayed Healing. Frontiers in Biosciences. 2004; 9: 283-89.

2. Mustoe TA, Cooter R, et al. International clinical guidelines for scar management. Plast Reconstr Surg. 2002;110: 560-72

3. Mendonça AC, Ferreira AS, Barbieri CH, Thomazine JA, Mazzer N. Efeitos do Ultra-som pulsado de baixa intensidade sobre a cicatrização por segunda intenção de lesões cutâneas totais em ratos. Acta Ortopédica Brasileira. 2006; 14: 152-57.

4. Posten W, Wrone A. Low-Level Laser Therapy for Wound Healing: Mechanism and Efficacy. Dermatologyc Surgery Inc. 2005; 31:334–340.

5. Medrado AP, Pugliese LS, Reis SR, Andrade ZA. Influence of low level laser therapy on wound healing and its biological action upon myofibroblasts. Laser in Surgery and Medicine. 2003; 32: 239-44.

6. Pereira PR, De Paula JB, Cielinski J, Pilonetto M, Von Bahten LC. Efeitos do laser de baixa intensidade em cultura bacteriana in vitro e ferida infectada in vivo. Rev. Col. Bras. Cir. 2014; 41(1): 049-055.

7. Reedy GK. Comparison of the photostimulatory effects of visible He-Ne and infared Gad-As lasers on healing impaired diabetic rat wounds. Laser in Surgery and Medicine. 2003; 33: 344-51

8. Karu T. Primary and secundar mechanism of action of visible to near-IR radiation cells. In: Journal Photochemical amd Photobiology. 1999; 49: 1-17.

9. Baxter D. Laserterapia de baixa intensidade. In: Kitchen S, editora. Eletroterapia: prática baseada em evidências. 11 ed. São Paulo: Manole; 2003. 171-90.

10. Kawamoto K, Senda N, Shimada K, Itol K, Hirano Y, Murai S. Antibacterial effect of yellow He-Ne laser irradiation with crystal violet solution on Porphyromonas gingivalis: an evaluation using experimental rat model involving subcutaneous abscess. Lasers Med Sci. 2000; 15:257–62.

11. Benvindo RG, Braun G, Carvalho AR, Bertolini GRF. Efeitos da terapia fotodinâmica e de uma única aplicação de laser de baixa potência em bactérias in vitro. Fisioter. Pesqui. 2008; 15(1): 53-57.

12. Lucas C, Criens-Poublon LJ, Cockrell CT, De Haan RJ. Wound healing in cell studies and animal model experiments by Low Level Laser Therapy; were clinical studies justified? A systematic review. Lasers Med Sci. 2002; 17(2):110-34.

13. Christiansen C, Desimone NA. Bactericidal Efect of 0.95-mW Helium-Neon and 5- mW Indium-Galium-Aluminum-Phosphate Laser Iradiation at Exposure Times of 30, 60, and 120 Seconds on Photosensitzed Staphylococus aureus and Pseudomonas aeruginosa InVitro. Physical Therapy. 1999; 79:839-846.

14. Coutinho F, Giordano V, Santos CM, Carneiro AF, Amaral NP, Touma MC, Giordano M. O efeito do laser de baixa energia no crescimento bacteriano “in vitro”. Rev Bras Ortop. 2007; 42(8):248-53.

15. Nussbaum EL, Lilge L, Mazzulli T. Effects of 630-, 660-, 810-, and 905-nm laser irradiation delivering radiant exposure of 1-50J/cm2 on three species of bacteria in vitro. J Clin Laser Med Surg. 2002; 20(6):325-33.

16. Chung AW, Petrofsky JS, Laymon M, Logoluso J, Park J, Lee J, Lee H. The effects of low level laser radiation on bacterial growth. Phys Ther Rehabil Sci. 2014; 3(1), 20-26.

17. Tiphlova O, Karu T. Action of low-intensity laser radiation on Escherichia coli. Crit Rev Biomed Eng. 1991; 18(6):387-412.

18. Thom KA, Schweizer ML, Osih RB, McGregor JC, Furuno JP, Perencevich EN, Harris AD. Impact of Empiric Antimicrobial Therapy on Outcomes in Patients with Escherichia coli and Klebsiella pneumoniae Bacteremia: A Cohort Study. BMC Infectious Diseases. 2008; 8:116.

19. Rodríguez CH, García S, Barberis C, Saposnik E, Weyland B, Nastro M, Losada A, Perazzi B, Almuzara M, Foccoli M, Vay C, Famiglietti A. Enterococcus spp.: Resistencia antimicrobiana en infecciones intrahospitalarias. Acta Bioquím Clín Latinoam, 2013; 47 (1):155-60.

20. Nussbaum EL, Lilge L, Mazzulli T. Effects of low-level laser therapy (LLLT) of 810 nm upon in vitro growth of bacteria: relevance of irradiance and radiant exposure. J Clin Laser Med Surg. 2003; 21(5):283-90.

21. Bisht D, Gupta SC, Misra V, Mital VP, Sharma P. Effect of low intensity laser radiation on healing of open skin wounds in rats. Indian J Med Res. 1994; 100:43-6.

22. Libanore DZ. Efeitos da terapia a laser de baixa intensidade (685 e 830nm) na taxa de proliferação bacteriana e na cicatrização de feridas cutâneas em modelo animal. Dissertação de Mestrado. Universidade de São Paulo - São Carlos, 2008.

23. Costa AF, Assis JCL. In vitro assessment of the bactericidal effect of low-power arsenium-gallium (AsGa) laser treatment. An Bras Dermatol. 2012; 87(4):654-6.

24. Sousa NTA, Guirro RRJ, Santana HF, Ssilva CCM. In Vitro Analysis of Bacterial Morphology by Atomic Force Microscopy of Low Level Laser Therapy 660, 830 and 904 nm. Photomedicine and Laser Surgery. 2012; May, 30(5): 281-285.

25. Maver-Biscanin M, Mravak-Stipetic M, Jerolimov V, Biscanin, A. Fungicidal Effect of Diode Laser Irradiation in Patients With Denture Stomatitis. Lasers in Surgery and Medicine. 2004; 35:259–262.

588167e27f8c9d710a8b45ba mtprehab Articles
Links & Downloads

Man. Ther., Posturology Rehabil. J.

Share this page
Page Sections